Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing.
نویسندگان
چکیده
منابع مشابه
Analysis of pre-mRNA splicing using HeLa cell nuclear extracts.
This protocol is used to determine the splicing behavior of pre-mRNAs in cell extracts that are capable of carrying out splicing (e.g., nuclear extracts from HeLa cells). (32)P-labeled RNA is incubated under splicing conditions for various times, and the resulting products are analyzed on denaturing polyacrylamide gels.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45427-0